Line-Shaped Illumination: A Promising Configuration for a Flexible Two-Photon Microscopy Setup

Line-Shaped Illumination: A Promising Configuration for a Flexible Two-Photon Microscopy Setup

Blog Article

An innovative two-photon microscope exploiting a line-shaped illumination has been recently devised and then implemented.Such configuration allows to carry out a real-time detection by means of standard CCD cameras and is able to maintain the same Stakes resolution as commonly used point-scanning devices, thus overcoming what is usually regarded as the main limitation of line-scanning microscopes.Here, we provide an overview of the applications in which this device has been tested and has proved to be a flexible and efficient tool, namely imaging of Greeting Cards biological samples, in-depth sample reconstruction, two-photon spectra detection, and dye cross-section measurements.These results demonstrate that the considered setup is promising for future developments in many areas of research and applications.